TABLE 1.
HIV-1 pNG-PRL library carrying combinations of zero to 11 amino acid substitutions
| Plasmid | No. of combinations of amino acid substitutions in protease | No. of independent clonesa | No. of clones containing a mutated protease fragment | % of clones with ligation efficiencyb |
|---|---|---|---|---|
| 4,096 | ||||
| pCAX-PRL | 4.5 × 106 | 3.4 × 106 | 76 | |
| pNG-PRL | 3.1 × 105 | 2.1 × 105 | 65 |
a
After transformation, a 1/1,000 volume of the cell suspension was inoculated on a Luria-Bertani agar plate containing 100 μg of ampicillin per ml incubated overnight. Numbers of independent clones were counted.
b
Ligation efficiency was obtained by >40 independent clones.