FIG. 3.
Interaction between STAT-3 and FPV of HBV enhancer 1. The EMSA was carried out in the presence of 32P-labeled FPV probe and lysates from Huh-7 cells transfected with v-Src. Lanes 1 and 6, 5 μg of lysates as a source of STAT-3; lanes 2, 4, and 7, 100-fold excesses of wild-type FPV, STAT-3, and PBF oligonucleotides, respectively; lanes 3, 5, and 8, 100-fold excesses of mutant FPV, STAT-3, and PBF oligonucleotides, respectively; lanes 11 and 12, protein-DNA complex incubated with anti-STAT-3 and anti-HNF-3 serum, respectively.