FIG. 6.

Cooperation of interaction between STAT-3 and HNF-3. (A) The EMSA was carried out in the presence of ³²P-labeled FPV probe. Binding of FPV probe with recombinant GST-HNF-3 (lane 1); with STAT-3 synthesized in bacteria (lane 2); both STAT-3 and increasing concentration of HNF-3 (lanes 3 and 4); same as shown in lane 4 but incubated with normal human IgG (lane 5); or with anti-HNF-3 serum (lane 6) and with anti-STAT-3 serum (lane 7). (B) STAT-3-HNF-3 complex interacts with STAT-3 oligonucleotide probe. The EMSA was carried out in the presence of ³²P-labeled STAT-3 oligonucleotide probe and nuclear lysates from HepG2 cells transfected with v-Src vector. Lane 1, probe alone; lane 2, 5 μg of lysates (source of STAT-3-HNF-3 complex); DNA-protein complex treated with normal human IgG (lane 3) or with anti-HNF-3 serum (lane 4).