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Cooperative activation of HBV Enh 1 by STAT-3 and HNF-3. Huh-7 cells were transiently transfected with 1 μg each of pFPV3Luc reporter plasmid and HNF-3 vectors. At 36 h after transfection, the cells were serum starved overnight and then stimulated with EGF (100 ng/ml) for 15 min. The cells were harvested and analyzed for luciferase activity. Each transfection was carried out in duplicate and repeated at least three times.
