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. 2002 Mar;76(6):2676–2682. doi: 10.1128/JVI.76.6.2676-2682.2002

FIG. 2.

FIG. 2.

FIG. 2.

FIG. 2.

Immunologic responses generated by various recombinant DNA vaccines. (A) Results of flow cytometry analysis of IFN-γ-secreting, E7-specific CD8+-T-cell precursors. Mice were immunized and splenocytes were collected and cultured as described in Materials and Methods. Note that vaccination of mice with MDV-1 VP22/E7 DNA generated a larger number of IFN-γ+ CD8+-T-cell precursors than did vaccination with any of the other constructs. The results shown are from one representative experiment of three that were performed. (B) Results of flow cytometry analysis of IFN-γ-secreting, E7-specific CD4+-T-cell precursors. (C) Results of an ELISA to determine E7-specific antibodies in the sera of the various vaccinated mice. Mice were immunized and sera were collected and cultured as described in Materials and Methods. O.D. (450), optical density at 450 nm.