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. 2026 Sep 22;46(10):BSR20250408. doi: 10.1042/BSR20250408

Figure 2. Activation of the AMPK pathway by MK-8722, SC4, and A-769662—long-term incubations.

Figure 2

HUVEC were treated with MK-8722 (MK), SC4, and A-769662 (A76) at the indicated concentrations for 24 h. Cells were lysed and subjected to Western blot analyses of ACC phosphorylated at S79 (A), Raptor phosphorylated at S792 (B), p70S6K phosphorylated at T389 (C), AMPK phosphorylated at T172 (D), and the corresponding non-phosphorylated proteins. Representative immunoblots and quantification by densitometry with normalisation of phosphorylated proteins to total proteins are presented. Mean + SD, n = 4. *P<0.05 versus non-treated control, #P<0.05 versus equimolar doses of SC4, and +P<0.05 versus equimolar doses of A-769662 using one-way repeated measurement ANOVA corrected by the Holm–Šidák method.