FIG. 3.

Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight with jacalin-conjugated agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.