FIG. 6.
Northern blot analysis of early, late, and very late transcripts from cells transfected with lef-11-null or control BACmids. Sf9 cells were transfected with either the lef-11-null BACmid (vAclef11KO), a control BACmid (vAc64−/+GUS), or the lef-11 repair BACmid (vAclef11KO-REP). At various times (12, 18, 48, or 72 h) posttransfection, total RNAs were isolated and used for Northern blot analysis with early (ie1), late (p6.9, p24, and gp16), or very late (p10) gene-specific probes. BACmids used for transfections are indicated at the top of the lanes, and gene-specific probes are indicated on the left. The sizes of expected RNAs from each gene-specific probe are indicated in kilobases on the right.