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. 2002 Mar;76(6):3072–3077. doi: 10.1128/JVI.76.6.3072-3077.2002

FIG. 2.

FIG. 2.

Flow cytometric analysis for surface expression of various chemokine receptors. Cells were washed with phosphate-buffered saline containing 2% fetal calf serum and incubated for 30 min with the following murine MAbs: anti-human CCR1 (clone 141), anti-human CCR2 (clone 48607.121; R&D Systems, Minneapolis, Minn.), anti-human CCR3 (clone 444.11), anti-human CCR4 (KM2160), anti-human CCR5 (clone 2D7; Pharmingen), anti-CCR6 (clone 53103.111; R&D Systems), anti-human CXCR4 (clone 12G5; DAKO, Kyoto, Japan), anti-human CXCR5 (clone 51505.111; DAKO), and anti-CX3CR1 (clone 2A9-1). The mouse primary antibodies were detected by using fluorescein isothiocyanate-conjugated sheep (Fab′)2 anti-mouse immunoglobulin G (Sigma). Dead cells were stained with propidium iodide to be gated out. Cells were analyzed on a FACSCalibur (Becton Dickinson), and the data were collected in the log mode. Ten thousand cells were analyzed per sample. Results from BCL-NU (an EBV-immortalized B-cell line) and Raji (an EBV-positive BL cell line) are shown as representative.