FIG. 3.
(Top panel) Northern analysis of HCV-specific RNA present in G418-resistant cell lines selected following transfection of Huh7 cells with the indicated replicon RNA. Lanes 1 and 2, synthetic RNA transcribed from pNNeo/3-5B (108 and 107 genome equivalents spiked into normal cellular RNA), respectively; lane 3, normal Huh7 cells; lanes 4 and 5, two independent, clonally isolated cell lines (cell lines 1 and 2) selected following transfection with NNeo/3-5B; lanes 6 to 9, other clonally isolated cell lines. (Lower panel) Northern analysis of β-actin mRNAs in the samples loaded into the gel shown in the top panel.