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G418-resistant cell colonies present 3 weeks after transfection with NNeo/3-5B and NNeo/3-5BΔi5A RNAs (20 μg of RNA per 10-cm-diameter culture dish) (top panel) or NNeo/3-5B(SI) and NNeo/3-5B(SI)Δi5A (1 μg of RNA per 10-cm-diameter culture dish) (bottom panel). Colonies were visualized by staining cells with crystal violet. wt, wild type.
