FIG. 2.
Mutant virus Sendai polymerase binding to nucleocapsids. VVT7-infected A549 cells were transfected with no plasmids (Mock) or the Sendai virus P and the indicated wt or mutant L plasmids. The cells were incubated overnight with Express-35S, and extracts were prepared. (A) Total samples were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). (B) Samples were incubated in the absence (−) and presence (+) of Sendai virus nucleocapsids (NC; 1 μg) and then pelleted through glycerol as described previously (11). The NC-associated proteins were analyzed by SDS-PAGE. The positions of the proteins are indicated.