FIG. 1.

Diagram of VSV/HIV recombinants and protein expression in infected cells. (A) The genomes of VSV-rwt and the three VSV/HIV recombinants are diagrammed in a 3′-to-5′ orientation on the negative-stranded viral RNA. Additional genes are expressed by duplication of the conserved VSV transcription start and stop signals (42). VSV-Gag and VSV-Env express the gag gene and the IIIb env gene, respectively, between the G and L genes of VSV. VSV-GagEnv contains both the gag and env genes in a single recombinant. (B) An SDS-polyacrylamide gel showing the expression of VSV and HIV proteins in rVSV-infected cells. BHK cells were infected with the recombinants shown at an MOI of 10 to 20. Four hours later, the medium was replaced with methionine-free medium containing [³⁵S]methionine. The cells were lysed 1 h later, and the extracts were fractionated by SDS-PAGE. The samples shown here were refractionated after normalization for VSV N protein content.