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E7 induces multinucleated cells in the suprabasal strata of raft cultures. Examples of binucleated and trinucleated cells in E7-transduced cultures. All cells were positive for 3H-TdR or BrdU, except those in panel b, which were positive for both. In panel a, one of the two binucleated cells did not incorporate any nucleoside analogue. Insets are enlarged views of selected nuclei (boxed). Arrows point to the basal stratum.
