Figure 1.
Growth inhibition by bile salts of three different strains of L. acidophilus. L. acidophilus strains 5C14E (A), 2G14E (B) and 2M14E (C) were grown to an OD600 nm of 0.6 (logarithmic phase) and 1 % inoculated in MRS broth containing or not 0.3 % oxgall in a microtiter plate. Readings at OD600 nm were taken at 15 min intervals during 12 h of incubation at 37°C in a Microplate Spectrophotometer System SpectraMax 340. Percentage of growth inhibition was calculated after 6 h as (1 – ABS/ACT) × 100.