FIG. 6.

Semiquantitative direct RT-PCR for confirmation of selected regulated Clontech genes. (A) Representative ethidium bromide-stained DNA products after agarose gel electrophoresis. Upper panel: control sample amplification of β-actin in uninfected and infected DMVEC RNAs, with IDV quantitation graphed to the right. Lower panel: test sample amplification of BMP4 in the same uninfected and infected DMVEC RNA samples, with IDV quantitation graphed to the right. (B) Histogram presentation of the relative IDV measurements for all 18 gene primer pairs examined, as shown in the example above. Uninfected RNAs are represented by open bars, and infected RNAs are represented by solid bars. Upper panel: KSHV-infected DMVEC in the absence of TPA. Lower panel: KSHV-infected DMVEC in the presence of TPA.