TABLE 1.
Gene | Atlas 1.2-I array intensity values
|
Atlas 1.2-II array intensity values
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
−TPA
|
R | +TPA
|
R | −TPA
|
R | +TPA
|
R | |||||
U | I | U | I | U | I | U | I | |||||
HSP27 | 40,060 | 36,580 | 1 | 32,600 | 29,770 | 2 | ||||||
60SRP13A | 44,040 | 37,480 | 2 | 28,740 | 30,720 | 5 | 33,320 | 31,700 | 2 | 33,410 | 32,570 | 4 |
Thymosin β10 | 34,120 | 34,220 | 3 | 36,215 | 34,800 | 1 | ||||||
Thymosin β4 | 31,060 | 27,600 | 4 | 30,670 | 28,100 | 3 | ||||||
Ubiquitin | 29,780 | 28,560 | 5 | 29,720 | 28,700 | 4 | 25,670 | 27,600 | 5 | 37,270 | 29,420 | 8 |
RhoGD1α | 28,460 | 30,500 | 6 | 27,040 | 26,050 | 7 | ||||||
mGST12 | 26,400 | 23,870 | 7 | 21,900 | 18,840 | 20 | ||||||
GST3 | 26,480 | 23,870 | 8 | 22,360 | 20,840 | 18 | ||||||
MCP1 | 26,460 | 17,900 | 9 | 11,610 | 12,020 | — | ||||||
DAD1 | 26,350 | 29,280 | 10 | 22,830 | 23,170 | 15 | ||||||
NKEnhF | 25,870 | 25,240 | 11 | 26,020 | 23,630 | 19 | ||||||
Gsα1 | 25,530 | 30,580 | 12 | 20,420 | 20,070 | 22 | ||||||
Mut-L (MLH1) | 25,600 | 26,620 | 13 | 19,460 | 17,840 | 24 | ||||||
Hyal-L2 | 25,430 | 25,450 | 14 | 28,160 | 30,720 | 8 | ||||||
Cathepsin-D | 25,360 | 26,400 | 15 | 20,630 | 23,050 | 21 | 17,970 | 24,500 | 19 | 24,370 | 25,120 | 13 |
Collagen 1α2 | 25,360 | 23,060 | 16 | 22,370 | 21,640 | 17 | ||||||
Parathymosin | 24,560 | 22,080 | 17 | 23,970 | 18,440 | 12 | ||||||
Calpain | 24,100 | 27,120 | 18 | 23,870 | 21,560 | 11 | 22,020 | 24,420 | 9 | 19,280 | 24,820 | 16 |
HLA-C4 | 23,820 | 29,500 | 19 | 25,630 | 27,140 | 10 | 21,060 | 27,920 | 12 | 28,980 | 31,080 | 5 |
40SRDS9 | 23,590 | 22,840 | 20 | 21,560 | 21,330 | 33 | ||||||
PUF | 23,400 | 28,370 | 21 | 26,730 | 25,100 | 9 | ||||||
BMP4 | 23,260 | 2,460 | 22 | 6,370 | 5,330 | — | ||||||
Amyloid α4 | 22,400 | 11,140 | 25 | 23,460 | 20,900 | 13 | 19,300 | 15,160 | 18 | 24,920 | 18,920 | 12 |
GADPH | 22,200 | 26,940 | 26 | 22,920 | 23,040 | 14 | 33,720 | 38,840 | 1 | 42,470 | 40,720 | 1 |
β-Actin | 21,400 | 24,000 | 29 | 22,630 | 21,820 | 16 | 28,720 | 21,200 | 3 | 42,470 | 43,580 | 2 |
TIMP1 | 21,020 | 36,630 | 30 | 28,940 | 38,870 | 6 | ||||||
Ferritin H | 27,960 | 28,320 | 4 | 22,860 | 23,900 | 14 | ||||||
Protein di-S isom | 25,120 | 27,000 | 6 | 32,440 | 28,930 | 3 | ||||||
VWF | 23,940 | 20,400 | 7 | 11,630 | 17,720 | — | ||||||
Clathrin AP50 | 21,920 | 23,500 | 8 | 29,070 | 22,920 | 6 | ||||||
Metallothionine | 22,290 | 21,700 | 10 | 4,220 | 15,280 | — | ||||||
CD81 | 21,860 | 22,400 | 11 | 28,550 | 26,700 | 7 | ||||||
Exodus 2 | 21,060 | 29,770 | 13 | 2,850 | 17,600 | — | ||||||
α1-Tubulin | 21,020 | 27,970 | 14 | 25,020 | 27,820 | 11 | ||||||
Annexin II | 20,820 | 20,100 | 15 | 21,070 | 16,120 | 15 | ||||||
Cofilin | 20,720 | 23,690 | 16 | 25,340 | 23,950 | 10 | ||||||
Gsα2 | 19,020 | 19,300 | 17 | 27,170 | 22,320 | 9 |
Hybridization intensity scores are given separately for each of the top 20 genes in the four Atlas 1.2-I array filter results obtained using cDNA probes prepared from uninfected and infected cell RNA (batch 1) in the absence and presence of TPA and similarly for each of the four Atlas 1.2-II array filters obtained using the same four-cDNA probe set. Averaged background values ranging from 11,780 to 16,540 on different filters have been subtracted, but the intensity values have not been normalized or mean adjusted. Numbers in bold type indicate significantly regulated values. Additional data for eight genes that are common to both filters are included. R, direct numerical rank order for each of the four uninfected DMVEC RNA filters. U = uninfected; I = infected.