TABLE 9.
Regulation | Gene name | RT-PCR fold regulation (RNA batch 2)
|
Gene array value
|
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Clontech (RNA batch 1)
|
Incyte (RNA batch 2)
|
|||||||||||
I/U | I+T/U+T | U+T/U | I+T/I | I+T/U | I/U | I+T/U+T | U+T/U | I+T/I | I/U | I+T/U+T | ||
Up | Angiopoietin 2 | +3.1 | −5.0 | +3.7 | +2.4 | +7.5 | — | — | — | — | +4.1 | −2.7 |
CD14 | +1.7 | +1.2 | +1.4 | −2.1 | −1.2 | +4.9 | NR | NR | NR | (+1.3) | NR | |
Exodus 2 | +19 | +18.4 | −3.8 | −3.2 | +4.8 | (+1.6) | +6.3 | −6.9 | −4.1 | — | — | |
IRF7 | +2.7 | +2.6 | +1.4 | +1.3 | +4.0 | +4.2 | +3.2 | NR | NR | (+1.4) | (+1.6) | |
Metallothionine H1 | +1.1 | +1.2 | +1.1 | +1.2 | +1.2 | NR | +7.6 | −6.3 | NR | — | — | |
Myx R1 | +17 | +80 | −2.5 | +32 | +32 | — | — | — | — | +7.7 | +26.4 | |
SISI3 | +18 | +25 | −3.7 | −2.0 | +9.2 | — | — | — | — | +7.4 | +2.4 | |
TGFβ3 | +6.7 | +3.5 | +1.1 | −1.8 | +3.8 | +2.0 | NR | NR | NR | +4.5 | +2.3 | |
TIMP1 | +9.0 | +2.8 | +3.2 | +3.6 | +15.3 | +3.2 | +3.6 | +2.8 | NR | — | — | |
RDC1 | +13.5 | +1.9 | +10.6 | +1.5 | +20.5 | +4.1 | +4.6 | NR | NR | +8.5 | +2.6 | |
Down | BMP4 | −145 | −5.0 | −345 | +2.1 | −69 | −7.1 | NR | −5.6 | NR | −4.7 | NR |
Connective tissue GF | −33 | −2.0 | −53 | −3.2 | −105 | NR | NR | +2.7 | +3.2 | −14.1 | −13.4 | |
Fibronectin | −21.6 | −10.6 | −10.0 | −4.9 | −106 | — | — | — | — | −12.6 | −13.6 | |
IL-8 | −50 | −3.9 | −35 | +5.5 | −9.1 | +2.1 | −4.6 | +5.4 | NR | NR | NR | |
α4-Integrin | −325 | −139 | −3.4 | −1.5 | −480 | — | — | — | — | −4.8 | −4.6 | |
MMP2 | −15.8 | −15.2 | +1.2 | +1.1 | −18 | NR | NR | NR | +3.2 | −5.1 | −5.2 | |
PAI-1 | −17.3 | −1.6 | −4.1 | +2.6 | −6.6 | −3.5 | +3.1 | −3.3 | +3.2 | −11.7 | −4.5 |
See Table 8, footnote a, except comparison was of real-time RT-PCR results with gene array values for batch 2 (Incyte) RNA in the absence and presence (+T) of TPA.