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Analysis of Pben509 activity in a ΔbenAB strain. Shown is β-galactosidase activity of DC253 carrying pDOW1019 (Pben509::lacZ) uninduced (0 mM benzoate) or induced with 5 mM sodium benzoate (A). Standard deviation is depicted by error bars. Cultures were grown in LB medium supplemented with uracil and proline. Benzoate metabolism in the parental strain (DC164, ΔpyrF) compared to the ΔbenAB strain (DC253, ΔpyrFΔbenAB) is shown in B.
