FIG. 5.

Intracellular distribution of vGCR protein in transiently transfected or Ad vector-transduced monolayer cells. Untagged expression vector plasmid DNA in plasmid pCJC398a containing the intact KSHV vGCR coding region under the control of the SV40 enhancer-promoter region was transfected into cells by the calcium phosphate precipitation procedure. Alternatively, DMVEC cultures were transduced with Ad-vGCR vector at an MOI of 10 PFU/cell for 15 days before fixing for IFA. (A) IFA with FITC-labeled anti-vGCR-N PAb in pSV2-vGCR-transfected Vero cells. (B) Immunohistochemistry with Vector Red-stained anti-vGCR-N PAb in pSV2-vGCR-transfected 293T cells. (C) IFA with FITC-labeled anti-vGCR-N PAb in Adv-GCR defective adenovirus vector-transduced DMVEC culture, with DAPI detection of cell nuclei.