FIG. 7.
Induction of vGCR protein by TPA in PEL cell lines as detected by IFA. Cell cultures were incubated in the presence of TPA for 48 h and fixed on lysine-coated glass slides by the formaldehyde-Triton X-100 procedure. Induced vGCR or vIL6 cytoplasmic and membrane proteins were detected with FITC-labeled anti-vGCR-N or anti-vIL6 rabbit PAb. (A) TPA-induced HBL6 cells (×100, vGCR PAb); (B) TPA-induced HBL6 cells (×100, vIL6 PAb); (C) TPA-induced JSC-1 cells (×60, vGCR PAb); (D) TPA-induced JSC-1 cells (×40, vIL6 PAb).