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Mapping phosphorylation sites of NSP5. SDS-PAGE analysis of immunoprecipitated, in vitro-phosphorylated GST-NSP5 mutant proteins (0.2 μg) incubated with (+) and without (−) cellular extract (cel. extr.) from Δ1/Δ3-transfected cells. GST protein negative control (−) (lane 17) was loaded without immunoprecipitation.
