Skip to main content
. Author manuscript; available in PMC: 2006 Feb 2.
Published in final edited form as: Virology. 2005 Sep 1;339(2):176–191. doi: 10.1016/j.virol.2005.05.031

Figure 2.

Figure 2

Fusion activity of nectin-1α/nectin-2α chimeras in a cell-mixing fusion assay. (A) Fusion assay. CHO-K1 cells expressing gB, gD, gH, gL, and T7 polymerase, or control plasmid pCAGGS substituted for gD plasmid (gD−), or all control plasmid (control) were mixed with CHO-K1 cells expressing nectin-1α or chimera and pG1NT7β-gal. Within each experiment, all values were expressed as a percentage of the value obtained for the positive control (nectin-1). (B). Cell-surface expression of chimeras in the fusion assay. CELISA analysis of receptor expressing cells from (A) using the nectin-1 mAb CK6. Within each experiment, all values were expressed as a percentage of the value obtained for the positive control (nectin-1). The absence of error bars for relative values given is due to standard deviations too small to generate a visible error bar. The fusion/CELISA experiments were performed three times and the mean values plus standard deviations for the combined relative results are depicted.

HHS Vulnerability Disclosure