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. Author manuscript; available in PMC: 2006 Feb 2.
Published in final edited form as: Virology. 2005 Sep 1;339(2):176–191. doi: 10.1016/j.virol.2005.05.031

Figure 5.

Figure 5

Binding of nectin-3:Fc, HSV-1(KOS) gD:Fc, or an anti-nectin-1 mAb to CHO-K1 cells expressing nectin-1α and nectin-1cyt−, or nectin-1gpi. CHO-K1 cells were transfected with plasmids expressing the receptors indicated, replated in 96 well plates and incubated in triplicate with nectin-3:Fc (A), gD:Fc (B), or Prr1-PE anti-nectin-1 mAb (C). The cells were then washed, fixed, incubated with biotinylated secondary antibodies, and an avidin-HRP detection system. Within each experiment, all values were expressed as a percentage of the value obtained for the positive control (nectin-1). The values graphed are the means and standard deviations of the relative results from three independent experiments.

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