Table 2.
Real-time, qPCR verification of GeneChip arrays results
| Affymetrix probe no. | Genes | Entrez gene no. | Fold change |
||
|---|---|---|---|---|---|
| GeneChip arrays | In vitro qPCR | In vivo qPCR | |||
| Up-regulated in M4A4 cells | |||||
| 2092_s_at | Osteopontin | 6696 | 41.79 | 80 | 25 |
| 809_at | RAB27A, member RAS oncogene family | 5873 | 2.15 | 5.2 | Equal |
| 41182_at | Tyrosinase-related protein 2 | 1638 | 3.35 | 3 | 2.1 |
| Up-regulated in NM2C5 cells | |||||
| 36911_at | Tyrosinase-related protein 1 | 7306 | 19.03 | 200 | 400 |
| 38772_at | Cysteine-rich, angiogenic inducer, 61 | 3491 | 13.82 | 100 | 4.5 |
| 681_at | Matrix metalloproteinase 8 (neutrophil collagenase) | 4317 | 5.8 | 80 | 20 |
| 115_at | Thrombospondin 1 | 7057 | 3.54 | 30 | 14 |
| 37951_at | Deleted in liver cancer 1 | 10395 | 3.34 | 2.4 | 7 |
| 37403_at | Annexin A1 | 301 | 5.24 | 9.2 | 7.6 |
| 36638_at | Connective tissue growth factor | 1490 | 7.51 | 7 | 10 |