Figure 4.
Histological analysis of pellets made from cells transduced with AdTGF-β1 at various MOIs, IGF-1 at 150 MOI, and a combination of AdTGF-β1 and AdIGF-1 at 150 MOI of each growth factor gene. AdTGF-β1 at 50 MOI (a), 100 MOI (b), 150 MOI (c). AdIGF-1 at 150 MOI (d), AdTGF-β1 and AdIGF-1 combination at 150 MOI of each growth factor gene (e), and control (f). All pellets were stained with toluidine blue. (Upper ×40, lower ×200 magnification.) Pellets made from cells transduced with AdTGF-β1 at 50 MOI show robust chondrogenic differentiation. There is no obvious chondrogenic differentiation in either AdIGF-1 or in combination with AdTGF-β1 in any of the pellets.