Table 1.
Micro-CT analysis of femurs in wild-type and mutant mice
| Genotype | Runx2-II+/+ | Runx2-II+/− | Runx2-II−/− |
|---|---|---|---|
| BV/TV (%) | |||
| 6 weeks | 21.2 ± 1.4 | 14.8 ± 1.4a (P = 0.0039) | 7.1 ± 1.4a,b (P = 0.0027) |
| 24 weeks | 14.5 ± 1.2c | 9.6 ± 1.3a,c (P = 0.0062) | – |
| (P < 0.0001) | (P < 0.0001) | ||
| Tb.N (mm−1) | |||
| 6 weeks | 6.44 ± 0.20 | 5.43 ± 0.21a (P = 0.0026) | 3.91 ± 0.26a,b (P = 0.0002) |
| 24 weeks | 4.65 ± 0.20c | 4.04 ± 0.21a,c (P = 0.0257) | – |
| (P = 0.0014) | (P = 0.0133) | ||
| Tb.Th (mm) | |||
| 6 weeks | 0.045 ± 0.001 | 0.041 ± 0.001a (P = 0.0333) | 0.035 ± 0.001a,b (P = 0.0343) |
| 24 weeks | 0.046 ± 0.002 | 0.041 ± 0.002a (P = 0.0050) | – |
| (P = 0.6158) | (P = 0.9855) | ||
| Tb.Sp (mm) | |||
| 6 weeks | 0.149 ± 0.010 | 0.184 ± 0.011a (P = 0.0280) | 0.266 ± 0.013a,b (P < 0.0001) |
| 24 weeks | 0.217 ± 0.011c | 0.254 ± 0.011a,c (P = 0.0090) | – |
| (P < 0.0001) | (P < 0.0001) | ||
| Ct.Th (mm) | |||
| 6 weeks | 0.18 ± 0.01 | 0.16 ± 0.01a (P = 0.0475) | 0.13 ± 0.01a,b (P < 0.0001) |
| 24 weeks | 0.241 ± 0.005c | 0.236 ± 0.005c (P = 0.4331) | – |
| (P < 0.0001) | (P < 0.0001) | ||
Data are mean ± SEM from 6 individual mice.
a
Significant difference from wild-type Runx2-II+/+ mice.
b
Significant difference from heterozygous Runx2-II+/− mice.
c
Difference between 6 and 24 weeks at the indicated P value using statistical analysis described in Materials and methods.