Table 2.
Biochemistry analysis of serum in wild-type and mutant mice
| Genotype | Runx2-II+/+ | Runx2-II+/− | Runx2-II−/− |
|---|---|---|---|
| Ca (mg/dl) | |||
| 6 weeks | 8.9 ± 0.16 | 8.8 ± 0.17 (P = 0.5921) | 9.3 ± 0.37 (P = 0.1856) |
| 24 weeks | 9.9 ± 0.27a | 9.6 ± 0.29a (P = 0.1856) | – |
| (P = 0.0028) | (P = 0.0194) | ||
| P (mg/dl) | |||
| 6 weeks | 10.5 ± 0.42 | 10.2 ± 0.44 (P = 0.6328) | 11.6 ± 0.95 (P = 0.1812) |
| 24 weeks | 9.5 ± 0.27a | 9.0 ± 0.26 (P = 0.3727) | – |
| (P = 0.0028) | (P = 0.1326) | ||
| Osteocalcin (ζg/ml) | |||
| 6 weeks | 451 ± 51 | 492 ± 49 (P = 0.2196) | 107 ± 56b,c (P = 0.0022) |
| 24 weeks | 114 ± 10a | 56 ± 9a,b (P = 0.0342) | – |
| (P < 0.0001) | (P < 0.0001) | ||
| OPG (ζg/ml) | |||
| 6 weeks | 2.9 ± 0.18 | 2.5 ± 0.18 (P = 0.0793) | 2.7 ± 0.25 (P = 0.4016) |
| 24 weeks | 2.4 ± 0.23 | 2.3 ± 0.24 (P = 0.6936) | – |
| (P = 0.0636) | (P = 0.4248) | ||
| RANK-L (pg/ml) | |||
| 6 weeks | 97.6 ± 5.1 | 66.4 ± 5.1b (P = 0.0003) | 28.3 ± 7.2b,c (P = 0.0003) |
| 24 weeks | 90.4 ± 4.5 | 59.9 ± 4.5b (P < 0.0001) | – |
| (P = 0.3021) | (P = 0.3462) | ||
| TRAP (U/L) | |||
| 6 weeks | 4.5 ± 0.35 | 3.0 ± 0.35b (P = 0.0078) | 2.6 ± 0.49b (P = 0.5163) |
| 24 weeks | 1.9 ± 0.18a | 0.99 ± 0.17a,b (P = 0.0160) | – |
| (P < 0.0001) | (P < 0.0001) | ||
Data are mean ± SEM. from 6 to 10 individual mice. Osteocalcin, OPG, and RANK-L are produced by osteoblasts, and TRAP is produced by osteoclasts.
a
Difference between 6 and 24 weeks at the indicated P value using statistical analysis described in Materials and methods.
b
Significant difference from wild-type Runx2-II+/+ mice.
c
Significant difference from heterozygous Runx2-II+/− mice at P < 0.05 by one-way ANOVA.