Figure 4.

Effect of cyclophosphamide upon vaccination with TC-1 cells that overexpress gp96.C57Bl/6 mice were subcutaneously injected with 2×10⁴ TC-1 cells. When tumors reached a diameter of 2mm, one group of mice (B) received an intraperitoneal injection of 2mg cyclophosphamide (CY). The other group (A) received PBS injections. Four days later, mice were vaccinated with 1×10⁶ TC-cells, mock-infected or infected ex vivo with Ad.Co (a bGal-expressing first-generation vector), Ad.mgp96, or Adsgp96 at an MOI of 500 pfu/cell. Growth of primary tumors was recorded over a period of 35 days. Mice were sacrificed when tumors reached a volume of 1000mm³ (for PBS injected groups) (C) or 200mm³ (for CY treated groups) and survival was recorded (D). The relatively high MOI of 500pfu/cell was chosen for TC-1 cell infection to inhibit outgrowth of vaccination tumors. Notably, Ad.Co infection at this MOI caused cell death within 5 days in cultured TC-1 cells.N=5 (Each line represents one mouse/tumor).