Fig. 3.

E2A drives transcription from a μE5-dependent reporter. a Schematic of the reporter construct (pGL/Δ56/μE5×3) that contains a minimal c-fos promoter (Δ56) with, upstream, a trimer of μE5 motifs. b Transcription driven from the reporter constructs by cotransfection of vectors expressing E2A (pRc/CMV/E2A) or CFEB1 (pRc/CMV/CFEB1) into the catfish B-cell line (1G8), T-cell line (G14D), or the mouse plasmacytoma (J558L). Expression is compared to the basal transcription assessed by cotransfection of an empty expression vector (pRc/CMV) with the reporter construct. Values are shown as mean±SD for six replicates