FIG. 3.

In vitro expansion of subdominant epitope-specific CTL from splenic T cells of virus-infected mice. Spleen cells from BeAn-infected mice at 8 days postinfection were stimulated for 5 days with the indicated peptide and used as effector cells in a 6-h ⁵¹Cr release assay. RMA-S cells loaded with stimulating peptide or unloaded cells were used as target cells. No lysis was observed against control peptides. Spontaneous release was less than 13%.