FIG. 5.

Determination of MHC class I restriction element for subdominant CTL epitopes. (A) Spleen cells from TMEV-infected mice were stimulated in vitro for 5 days with the VP2_165-173 peptide and used as effector cells to assess lytic activity against L929-K^b or L929-D^b target cells loaded with the stimulating peptide. (B) CNS-IL were assayed ex vivo for the ability to lyse VP3_101-120-loaded L929-K^b and L929-D^b target cells. Spontaneous release was similar for both target cell lines used (≈10 to 15%).