Fig. 3.

TUNEL staining of the hippocampus in wild type and GalR1 KO mice following SE. Animals were euthanized 4 days after SE. TUNEL-positive cells appear green, DAPI-counterstained nuclei are blue. A, C, E, G, I- Wild type (+/+); B, D, F, H, J- GalR1 KO (-/-) mice. Li-pilocarpine SE (A-D) led to moderate injury in the CA1 pyramidal cell layer in WT animals (A), and significantly more severe injury in GalR1 KO mice (B see also Fig. 4b). No injury to hilus of the dentate gyrus was observed in WT (C), while GalR1 KO animals showed moderate-to severe injury to hilar interneurons; in addition few cells located in dentate granule cell layer (arrows) were TUNEL-positive (d). PPS-induced SE (E-J) led to moderate injury to CA1 in both wild type and GalR1 KO mice D no differences between the groups (E,F, also see Fig. 4B. No hilar injury was observed on sides contralateral (contra, G) and ipsilateral (ipsi, I) to PPS in WT. In GalR1 KO mice mild injury to hilar interneurons was seen on the PPS side (ipsi, J), but not on the contralateral side (contra, H). Scale bar 50 micron.