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. Author manuscript; available in PMC: 2006 Feb 2.
Published in final edited form as: Nature. 2005 Jul 21;436(7049):415–419. doi: 10.1038/nature03798

Figure 4.

Figure 4

Structure-based mutational analysis of Rab and Rabeonsoyn-5 interaction specificity. a, Local alignment of representative Rab GTPases and Rabenosyn-5 homologs. Residues within the binding interfaces for Rab22-Rbsn728–784, Rab4-Rbsn440–503, Rab5-Rabaptin-522, and Rab3-Rabphilin21 are highlighted according to conservation. b, Effect of site specific substitutions on the interaction between Rab5 and Rbsn728–784 or Rab4 and Rbsn440–503. Mean values and standard deviations were calculated from 2 experiments. c, Views of the Rab22-Rbsn728–784 and Rab4-Rbsn440–503 interfaces relevant to the mutations discussed in the text. Also shown (middle panel) is the structure of the Rab5 G55Q mutant following superposition with Rab22.