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. 2006 Jan;5(1):155–166. doi: 10.1128/EC.5.1.155-166.2006

FIG. 6.

FIG. 6.

Coexpression of DA Cdc42 in DN Ras mutants promotes spore germination. (A) Phenotypes of the double mutant expressing DA Cdc42 and DN Ras. Spores of the wild type (panel 1), the DA Cdc42 mutant (panel 2), the DN Ras DA Cdc42 double mutant (panel 3), and the DN Ras mutant (panel 4) were inoculated onto minimal medium supplemented with 15 mM quinic acid, and spore germination was observed by light microscopy 24 h postinoculation. (B) Relative spore germination rates in different strains. Spores from the wild type, the DA Cdc42 mutant, DN Ras mutant, and the double mutants (DN Ras DA Cdc42) were plated at about 100 spores per plate on minimal medium amended with 15 mM quinic acid, and the numbers of germinating spores were recorded after 24 h postinoculation. The percent germination rate represents the percentage of germinating spores compared to the total number of inoculated spores. Error bars represent standard derivations. Experiments were repeated three times, and representative data are shown. (C) Hyphal morphologies of different mutants. Spores of indicated strains were separately inoculated onto minimal medium and grown at room temperature for 6 days. Images depict hyphal morphologies on minimal medium. 1, wild type; 2, DA Cdc42 mutant; 3, DN Ras DA Cdc42 double mutant; 4, DN Ras mutant.