TABLE 2.
MVE-specific antibody responses in C57B1/6 wt mice and in mutant mice deficient in granule exocytosis and/or Fas pathways of cytotoxicity
| Expt no. | Virus dose (PFU) | Mouse strain | Time (days p.i.) | No./group | % Seroconversion | Mean antibody titer,a log10 (range) |
|---|---|---|---|---|---|---|
| 1 | 0.1 | Wild type | 10 | 6 | 100 | 3.7 (2.6-4.1) |
| perf−/− | 3 | 100 | 4.0 (3.8-4.1) | |||
| gzmAxB−/− | 4 | 100 | 4.2 (4.1-4.4) | |||
| gld | 3 | 100 | 3.7 (2.6-4.4) | |||
| Wild type | 21-25 | 4 | 100 | 4.3 (3.8-4.7) | ||
| perf−/− | 3 | 100 | 4.6 (4.1-5.0) | |||
| gld | 1 | 100 | 5.0 | |||
| 102 | Wild type | 21-32 | 11 | 100 | 4.0 (2.9-4.7) | |
| perf−/− | 4 | 100 | 4.3 (4.1-4.4) | |||
| gzmAxB | 5 | 100 | 3.8 (3.5-4.1) | |||
| perf × gzmA × B−/− | 8 | 100 | 4.0 (3.8-4.1) | |||
| gld | 8 | 100 | 4.5 (3.5-5.0)b | |||
| lpr | 4 | 100 | 4.3 (3.8-4.7) | |||
| 2 | 105 | Wild type | 5 | 6 | 100 | 2.5 (2.0-2.9) |
| perf−/− × gld | 3 | 100 | 2.7 (2.6-2.9) | |||
| Wild type | 10 | 7 | 100 | 3.4 (3.2-3.5) | ||
| perf−/− × gld | 4 | 100 | 4.0 (3.8-4.4)b | |||
| Wild type | 15 | 7 | 100 | 3.7 (3.5-3.8) | ||
| perf−/− × gld | 4 | 100 | 4.1 (3.5-4.7) | |||
| Wild type | 21 | 7 | 100 | 3.8 (3.5-4.1) | ||
| perf−/− × gld | 4 | 100 | 4.5 (4.1-4.7)b | |||
| gld | 3 | 100 | 4.5 (4.4-4.7)b | |||
| perf−/− | 3 | 100 | 4.0 (3.8-4.1) |
a
MVE-specific antibody titers were determined by ELISA, and endpoints were calculated as described in Materials and Methods.
b
Statistically significant difference (P ≤ 0.05; Mann-Whitney test) relative to groups of wild-type mice.