FIG. 5.

FACS analysis of the effect of α and β RNAi knockdown on the mitochondrial membrane potential. Analysis of 2.5 × 10⁶ cells/ml each of wild type (WT) and RNAi-induced cells stained with a 2.5 μM concentration of the membrane potential sensitive MitoTracker Red is shown. CMXRos (red trace of each panel) and treated with 50 μM CCCP prior to MitoTracker staining (green trace of each panel) were analyzed with the FACSCalibur flow cytometer to monitor the changes in fluorescence intensity. The y axis of the graphs represents the total cell counts (×10) per sample, and the x axis indicates the relative fluorescence intensity of the samples. (A) Relative fluorescence intensity of wild-type stained cells. (B) Relative fluorescence intensity of β RNAi-induced cells. Arrows point to peak 1 (high fluorescence) and peak 2 (low fluorescence) of the β RNAi cells. (C) Relative fluorescence intensity of α RNAi-induced cells from clone 8. (D) Relative fluorescence intensity of α RNAi-induced cells from clone 11. Blue shaded peak, background intensity.