FIG. 2.

Effect of TbPLK depletion on the growth of procyclic-form T. brucei. Cloned procyclic T. brucei cells harboring the TbPLK RNAi plasmid construct were incubated in a culture medium consisting of 1 μg/ml tetracycline (+Tet) or without teracycline (−Tet). Growth of the cells was monitored daily, and the numbers of cells were plotted on a logarithmic scale. The inset shows the level of TbPLK mRNA on the third day of RNAi measured by semiquantitative RT-PCR on the RNA sample isolated from the cells. α-Tubulin (TUB) mRNA was included as a sampling control.