FIG. 7.

Overexpression of TbPLK-3HA. (A) TbPLK-3HA expression was induced by adding 0.1 μg/ml tetracycline (Tet) to the culture medium. Cell samples were collected 5 days thereafter, and total RNA and protein prepared from the cells were analyzed and quantitated by semiquantitative RT-PCR (top inset) and Western blotting (bottom inset), respectively. Samples of cells were counted and plotted versus time. (B) Cells harvested after 5 days of induction were fixed and stained with DAPI for numbers of nuclei and kinetoplasts in individual cells. Data are presented as mean percentages ± standard errors of total cells counted (>200) from three independent experiments. The numbers above the bars represent percentages of the cell population.