FIG. 8.

Localization of TbPLK in procyclic-form T. brucei. Cells transfected with the overexpression vector containing TbPLK-3HA were treated with tetracycline (0.1 μg/ml) for 5 days. The cells were harvested, fixed, and used to coat poly-l-lysine coverslips for immunostaining. Immunostaining of basal bodies (BB) is in blue, and that of TbPLK-3HA is in green. Nuclei (N) were stained with DAPI 9 (red). Images were first processed with Imagej 1.33u (NIH) and then modified with Adobe Photoshop CS. K, kinetoplast; IF, immunofluorescence images. Bars, 5 μm.