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TbPLK is apparently localized in the FAZ. The cytoskeleton and flagella were prepared from TbPLK-3HA-expressing procyclic-form T. brucei cells and underwent immunofluorescence assays. (A) Immunolocalization of TbPLK-3HA in isolated cytoskeletons. (B) Immunolocalization of TbPLK-3HA in high-salt-treated cytoskeletons. F, flagellum; N, nucleus; K, kinetoplast. Bars, 5 μm.
