FIG. 5.

Cellular localization of TL and TLF taken up by wild-type T. b. brucei 427 (A to D) and T. b. brucei 427-SRA-Ty (E to H). The positions of the nucleus and the kinetoplast were visualized by staining with DAPI (blue). (A and E) Cells visualized by bright-field (BF) microscopy overlaid with DAPI-stained DNA. Cells are shown following incubation with Alexa-conjugated TLF (red) (B and F) or Alexa-conjugated TL (green) (C and G). (D and H) Overlays of images of cells stained for DNA and visualized for the appearance of Alexa-conjugated TLF and TL. The arrows in panel D indicate the sites of TLF and TL colocalization in wild-type T. b. brucei.