TABLE 1.
Real-time RT-PCR primers used in this studya
| orf6 | orf19 | Gene | Primer name | Sequence (5′-3′) |
|---|---|---|---|---|
| orf6.9037 | orf19.6000 | CDR1 | qCDR1-2F | 5′ AGG CAA TTA GTC AAG ACT CTT CTT CA 3′ |
| qCDR1-3R | 5′ AAG TAT TTC AAT AAA CCT GCT GAC GAG 3′ | |||
| CDR2b | qCDR2-4F | 5′ TGC AAA AGT CAC TTG TGC ACC TA 3′ | ||
| qCDR2-4R | 5′ TGA ACA AGT ACC ATC ACT ATT TGT GGA 3′ | |||
| TEF1c | qRTTEF1F | 5′ CCA CTG AAG TCA AGT CCG TTG A 3′ | ||
| qRTTEF1R | 5′ CAC CTT CAG CCA ATT GTT CGT 3′ | |||
| ALS4c | qRTALS4-F | 5′ TCT GCA ACA CGA GTC AGC TCA 3′ | ||
| qRTALS4-R | 5′ CCG CAC CAA CAC AAG CAT ATA T 3′ | |||
| EBP1d | qEBP1-F | 5′ ATC CTA ATG CTG CTA AAC ATG CAC 3′ | ||
| qEBP1-R | 5′ GCC AAT CTA TTA GCA CCA ACA ACT T 3′ | |||
| orf6.6398 | orf19.1429 | SOH1 | q1429-F | 5′ TCT TTG ATG AAT GAC ATG GTT AAG C 3′ |
| q1429-R | 5′ CTT GGT CAT TCG CAT TCA GTT G 3′ | |||
| orf6.3437 | orf19.1172 | PHO84 | q1172-F | 5′ AAG CTG CCA TTG ATG TCC ATG 3′ |
| q1172-R | 5′ AAG AAG CCT TTG GTG GAG CA 3′ | |||
| orf6.6993 | orf19.6844 | ICL1 | q6844-F | 5′ TGC TTC CAC TTC TAA CGA ACC AT 3′ |
| q6844-R | 5′ TCC ATA GGA TAA TCA GCC AAA TCT G 3′ | |||
| orf6.3288 | orf19.3618 | YWP1 | orf-3618F | 5′ ATT CCA GCT TCA TTA GCT GCT TTC T 3′ |
| orf-3618R | 5′ ATA TTC TTC AAG GGA AAC AAA TGT GA 3′ | |||
| orf6.6934 | orf19.3111 | PRA1 | q3111-F | 5′ GCG ACA GTG GCT CTG ATT CA 3′ |
| q3111-R | 5′ GAT GAG AAC TTG AGG CTG TGC TAC T 3′ | |||
| orf6.648 | orf19.1027 | PDR16 | q1027-F | 5′ GAG CTA CTA AAT GGC ATG AAT CTG AG 3′ |
| q1027-R | 5′ GGT TCG GAT ATA CCA AAT TCA CGT 3′ | |||
| orf6.3947 | orf19.909 | STP4 | q909-F | 5′ CCT GTG TCA AGA CCA AGA AGT ACG T 3′ |
| q909-R | 5′ ATT AGA CAG TAT AGC TGG ATG CAG TTG 3′ | |||
| orf6.5917 | orf19.23 | RTA3 | qRT-1923F | 5′ CAA TGA AGA ATC GAC GTT GCA 3′ |
| qRT-1923R | 5′ TCA TTC CTT ATT CTC TTG AAT AGG ATC AC 3′ | |||
| orf6.854 | orf19.3188 | TAC1 | qRT-3188F | 5′ GAA AGA TCG TGT TAA AGA TGC CAA C 3′ |
| qRT-3188R | 5′ GAG AGA AAA TGC AAG TAA TTG AAT GAG TAC 3′ |
a
Gene sequences were obtained from the C. albicans genome annotation data at http://agabian.ucsf.edu/canoDB/.
b
The CDR1 probe on the microarray hybridizes to both CDR1 and CDR2, so gene-specific real-time RT-PCR primers were designed to differentiate between the signals.
c
Primers were defined by Green et al. (21). The ALS4 probe on the array hybridizes to both ALS2 and ALS4 so signals must be differentiated from each other using gene-specific real-time RT-PCR primers.
d
EBP1 is not represented on the microarray. EBP1 gene expression data were derived using real-time RT-PCR.