TABLE 1.

Relative frequencies of homologous recombination (HR) in wild-type and akuAΔ genetic backgrounds of A. fumigatus as estimated from targeted replacement of the abr2 locus^a

Strain	% HR at arm length:
	∼2.0 kb	1.5 kb	1.0 kb	0.5 kb	0.1 kb
Wild type	22	14	10	2	0
akuAΔ	95	96	96	(84)	(75)

^aFrequencies were assessed from transformation experiments with strains D141 and AfS28 as recipients; transformation protoplasts were mixed with 10 μg of DNA to achieve polyethylene glycol-mediated fusion (12). Percentages were calculated from the proportion of brownish colonies among the primary transformant pool by scoring 50 randomly chosen colonies in total; values in parentheses were estimated from fewer transformants (19 and 4 for 0.5 and 0.1 kb, respectively) and therefore have to be considered statistically insignificant.