TABLE 3.
Sporulation in S288C strain background
| Strain | Relevant genotype | % Spore formationa |
|---|---|---|
| Y315 | GCS1 | 69.3 ± 4.0 |
| Y4403 | gcs1Δ | <0.1 |
| Y5408 | gcs1R54K | <0.1 |
| Y5040 | spo14Δ | <0.1 |
| Y5492 | gcs1Δ spo14Δ | <0.1 |
| Y5664 | arl1Δ | 46.3 ± 9.8 |
| Y4690 | GCS1-GFP | 41.7 ± 3.8 |
| Y4804 | GCS1PHΔ-GFP | 62.7 ± 8.4 |
| Y5396 | gcs1R54K-GFP | <0.1 |
| Y5328 | gcs1R54K-GFP × GCS1 | 70.7 ± 5.4 |
| Y5609 | GCS1 SNF7-GFP | 75.6 ± 8.6 |
| Y5617 | gcs1Δ SNF7-GFP | <0.1 |
a
A minimum of 300 cells were counted per liquid culture over a minimum of three independent experiments. The final % sporulation includes cells that contained two, three, or four mature spores. Values are means ± SD from three independent experiments.