TABLE 5.
Analysis of endocytosis by FM4-64 in sporulating cells
| Strain and time of analysis (min) | Relevant genotype | % FM4-64 signala
|
||
|---|---|---|---|---|
| Punctate | Vacuoleb | No signal | ||
| Y315 | GCS1 SPO14 | |||
| 0 | 59.4 | 3.1 | 37.5 | |
| 10 | 31.0 | 41.0 | 28.0 | |
| 20 | 4.4 | 80.6 | 15.0 | |
| 30 | 1.1 | 76.6 | 22.3 | |
| 60 | 0.0 | 82.9 | 17.1 | |
| Y4403 | gcs1Δ | |||
| 0 | 27.5 | 34.5 | 38.0 | |
| 10 | 32.1 | 10.2 | 57.7 | |
| 20 | 46.6 | 21.9 | 31.5 | |
| 30 | 25.3 | 22.8 | 51.9 | |
| 60 | 32.5 | 29.6 | 37.9 | |
| Y5040 | spo14Δ | |||
| 0 | 83.2 | 3.4 | 13.4 | |
| 10 | 94.5 | 1.6 | 3.9 | |
| 20 | 46.1 | 30.9 | 23.0 | |
| 30 | 1.1 | 95.2 | 3.7 | |
| 60 | 0.0 | 97.7 | 2.3 | |
| Y5492 | gcs1Δ spo14Δ | |||
| 0 | 8.6 | 38.9 | 52.5 | |
| 10 | 23.8 | 41.4 | 34.8 | |
| 20 | 12.7 | 27.6 | 59.7 | |
| 30 | 8.6 | 26.8 | 64.6 | |
| 60 | 9.1 | 38.8 | 52.1 | |
| Y5408 | gcs1R54K | |||
| 0 | 8.4 | 33.7 | 57.9 | |
| 10 | 26.8 | 25.6 | 47.6 | |
| 20 | 32.0 | 23.2 | 44.8 | |
| 30 | 30.7 | 23.2 | 46.1 | |
| 60 | 28.6 | 27.8 | 43.6 | |
a
Localization of FM4-64 to punctate or vacuolar structures within cells. This experiment was performed multiple times revealing similar results. A minimum of 300 cells was counted in each experiment. These numbers reflect one individual experiment.
b
Cells showing localization of FM4-64 to normal or fragmented vacuoles.