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TNF-α and IL-1β dramatically increase NF-κB DNA binding in Ad12-transformed cells. 12-1 cells were treated by using the same conditions as those described for Fig. 2. Nuclear extracts were analyzed by EMSA for NF-κB DNA binding activity to the R1 probe. Positions for p50/p50 homodimers and p65/p50 heterodimers are indicated. F, free probe.
