Figure 6.

Overall stability, stacking and base pairing contributions for DNA polymers at different temperatures (a) Temperature dependence of the stacking contributions to the stability of A•T- (red circles) and G•C- (blue circles) containing polymers. Straight solid line of the same color gives the temperature dependence of the stability parameter of corresponding polymer calculated using Equations 5 and 6 at [Na⁺] = 15 mM. (b) Temperature dependence of A•T (red) and G•C (blue) base pairing parameters calculated as a difference between stability and stacking terms using data in (a). Horizontal broken lines correspond to mean values of $\Delta G_{\text{A}•\text{T}}^{\text{BP}}=0.57$ kcal/mol and $\Delta G_{\text{G}•\text{C}}^{\text{BP}}=-0.11$ kcal/mol.