FIG. 4.

RPA of cytoplasmic and nuclear RNAs from transfected 293 cells with or without 24 h of LMB treatment reveals that LMB augments cytoplasmic accumulation of SNV RU5 gag RNA. (A) Relationships among the gag reporter plasmid, an antisense HIV-1 5′ UTR RNA probe, and protected unspliced and spliced transcripts with sizes indicated. ss, splice site; nt, nucleotides. (B) RPA and PhosphorImager analysis of cytoplasmic (30 μg) and nuclear (15 μg) RNAs. Labels indicate the reporter plasmid, LMB treatment, and protected transcript. (C) Northern blot analysis of 10-μg aliquots of the RNA samples hybridized to an actin probe.