TABLE 4.

Effects of Rev on protein and RNA levels

Plasmid and transcript	Minus Rev				Plus Rev				Fold increasec
	Gag concn (ng/ml)	Nuclear RNAa	Cytoplasmic RNA	TEb	Gag concn (ng/ml)	Nuclear RNA	Cytoplasmic RNA	TE
pYW100RRE
Unspliced	219	11,599	1,194	0.18	2,985 (14)d	24,522	11,388	0.26	4.5
Spliced	NAe	14,236	10,675	NA	NA	12,405	10,417	NA	1.1
pTR155
Unspliced	33	1,399	452	0.07	1,391 (42)	5,037	4,819	0.30	2.9
Spliced	NA	858	1,395	NA	NA	1,840	2,777	NA	0.9

^aRNA levels were determined by RNase protection assay and are expressed in PhosphorImager units and normalized to the GAPDH signal.

^bTE, translational efficiency (ratio of Gag protein production to cytoplasmic RNA level).

^cRatio of cytoplasmic RNA to nuclear RNA in the presence and absence of Rev.

^dFold increase in response to Rev.

^eNA, not applicable.